Name: Firefly luciferase

Source: Recombinant Escherichia coli

Molecular weight: approximately 62KD

Structure: Approximately 62KDa monomer (SDS-PAGE)

Specific activity: ≥ 3.5 × 1011cpm/mg protein

Purity: greater than 95%

The optimal reaction pH is 7.0-8.5

PH stability: 6.0-9.0

Thermal stability: 20 ℃ for 3 hours; -20 ℃ for 12 months and -70 ℃ for 24 months.

Instructions for use: Dissolve (Tris/HCl 20mM pH 7.5, NaCl 500mM; Glycerol 20%; DTT 1mM； Dissolve at a final concentration of 4mg/mL.

Note: Dissolve 2mg/bottle of enzyme dry powder in 500uL solution. If further application requires freeze-drying, please do not add glycerol to the reconstitution solution.

-Store at 20 ℃ -70 ℃ to avoid repeated freezing and thawing.

Application: To detect the ATP content of various samples.

1. Lane 2 and 3 are firefly luciferase preparations produced by Shanghai Guchen Company, and lane 4 is

The protein molecular weight standards, from top to bottom, are 97400, 66200, 400300, and 31000, respectively,

2010014400 (Dalton). 5. 6 and 7 lanes are produced by Promega Corporation in the United States

QuantiLumRecombinant Luciferase preparation. 1. 2

The sample addition amounts for lanes 3, 7, 6, and 5 are 2.5 micrograms, 3.8 micrograms, and 7.5 micrograms, respectively.