CAS：2591-17-5

Introduction:

D-Luciferin is a substrate for the adenosine triphosphate (ATP)-dependent bioluminescence reaction. The principle of bioluminescence is that luciferin is oxidized by luciferase in the presence of ATP and oxygen. The chemical reaction equation is as follows: ATP + D-Luciferin + O2 → Oxyluciferin + AMP + PPi + O2 + Light.

Mechanism of action

The mechanism of action of D-luciferin involves the oxidation and luminescence of luciferin (the substrate) under the influence of ATP and luciferase. When there is an excess of luciferin, the number of photons generated is positively correlated with the concentration of luciferase.

Application(s)

D-Luciferin is a substrate for the adenosine triphosphate (ATP)-dependent bioluminescence reaction. The bioluminescence reaction of luciferin/luciferase is commonly used for the detection of ATP, metabolites that can be converted into ATP (such as AMP, ADP, cAMP), and enzymes that can generate ATP (such as creatine kinase). Therefore, the bioluminescence reaction can be applied to the detection of a wide range of biological materials.

Biological activity

D-Luciferin (Firefly luciferin) is a popular substrate for bioluminescence in the presence of ATP, used for luciferase-based bioluminescence imaging and cell-based high-throughput screening.

In vitro study

D-luciferin reacts with luciferase, ATP, and oxygen to emit light, which is detected by sensitive photographic film, thereby visualizing antibodies bound to alkaline phosphatase. In in vivo studies conducted on immunocompetent mouse models with ovarian cancer, the use of D-luciferin substrate and firefly luciferase preserved tumor-host immune interactions, as the bioluminescence procedure provided a more sensitive indicator of tumor growth compared to body weight gain.