CAS：103404-75-7

Introduction:

D-Fluorescein sodium is a hygroscopic orange-red powder that dissolves in water to form a yellow solution with yellow-green fluorescence, which disappears upon addition of acid; however, it reappears when added to neutral or alkaline solutions.

Application(s)

D-Luciferin sodium can be used as an indicator to confirm the source of groundwater, determine the approximate volume of water flowing out from cracks, search for sources of drinking water pollution, study the infiltration of industrial wastewater into soil, and serve as an indicator for corneal trauma.

Preparation

It is produced by condensing resorcinol with phthalic anhydride in the presence of zinc chloride or sulfuric acid, and then converting it into a sodium salt.

Biological activity

D-Luciferin (D-(-)-Luciferin, Firefly luciferin) sodium salt is the natural substrate of luciferase, which catalyzes the production of light by bioluminescent insects.

Purpose/Use

D-Luciferin sodium can be used for imaging analysis of luciferase-labeled genes and luciferase-fusion genes expressed in vivo/in vitro in living cells, tissues, or organisms; 2) it is widely used in reporter gene analysis, immunoassay, and ATP fluorescence hygiene monitoring analysis.

Purpose/Use

A heterocyclic luminescent compound, which is a natural ligand for luciferase used to detect cell activity, can catalyze bioluminescence in insects to produce typical yellow-green light.